Article
Effects of cold ischemia and inflammatory tumor microenvironment on detection of PI3K/AKT and MAPK pathway activation patterns in clinical cancer samples.
International journal of cancer - 1 Oct 2012
Bonnas Christel, Specht Katja, Spleiss Olivia, Froehner Stefanie, Dietmann Gabriele, Krüger Juliane M, Arbogast Susanne, Feuerhake Friedrich
Abstract excerpt
The accuracy of common markers for PI3K/AKT and MAPK pathway activation in preclinical and clinical cancer biomarker studies depends on phosphoepitope stability and changes of phosphorylation under ischemia. Herein, we define conditions under which phosphoepitope-specific duplex immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tumor tissues reflects pathway activation in situ as accurately as...
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