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Article

Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry Enables Multiplex, Quantitative Pharmacodynamic Studies of Phospho-Signaling.

Molecular & cellular proteomics : MCP - 1 Aug 2015

Whiteaker Jeffrey R, Zhao Lei, Yan Ping, Ivey Richard G, Voytovich Uliana J, Moore Heather D, Lin Chenwei, Paulovich Amanda G

Abstract excerpt

In most cell signaling experiments, analytes are measured one Western blot lane at a time in a semiquantitative and often poorly specific manner, limiting our understanding of network biology and hindering the translation of novel therapeutics and diagnostics. We show the feasibility of using multiplex immuno-MRM for phospho-pharmacodynamic measurements, establishing the potential for rapid and precise...

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Identifiers

PMID: 25987412
DOI: 10.1074/mcp.O115.050351
PMCID: PMC4528251

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ATM

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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