Article
Effect of non-ionic detergents on apparent enzyme mechanism: V121A mutant of Streptomyces cholesterol oxidase endowed with enhanced sensitivity towards detergents.
Protein engineering - 1 Aug 1998
Nishiya Y, Yamashita M, Murooka Y, Fujii I, Hirayama N
Abstract excerpt
One of the mutants of Streptomyces cholesterol oxidase with the Val121Ala mutation (V121A) was kinetically analysed. Although the reaction rate-substrate concentration curve of wild type follows a simple Michaelis-Menten equation, that of V121A is sigmoidal. The cooperativity was apparent and cau...
Topics
- Binding Sites
- Cholesterol
- Cholesterol Oxidase
- Detergents
- Enzyme Stability
- Kinetics
- Models, Molecular
- Mutation
- Octoxynol
- Protein Conformation
- Streptomyces
- X-Ray Diffraction