Article
Allele-specific real-time PCR testing for minor HIV-1 drug resistance mutations: assay preparation and application to reveal dynamic of mutations in vivo.
Chinese medical journal - 1 Dec 2010
Guo Dong-Xing, Li Han-Ping, Li Lin, Zhuang Dao-Min, Jiao Li-Yan, Wang Zheng, Bao Zuo-Yi, Liu Si-Yang, Liu Yong-Jian, Li Jing-Yun
Abstract excerpt
BACKGROUND: It is very important for the clinical management to test for minor HIV-1 resistance mutations accurately and sensitively. The conventional genotypic assays of HIV drug resistance detection based on sequencing can only discriminate the mutations which present in more than 20% - 30%. The aim of this study was to evaluate allele-specific real-time PCR (ASPCR) to detect the resistance-related mutations...
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