Article
Identification of cis elements necessary for glucocorticoid induction of growth hormone gene expression in chicken embryonic pituitary cells.
American journal of physiology. Regulatory, integrative and comparative physiology - 1 Mar 2012
Heuck-Knubel Kristina, Proszkowiec-Weglarz Monika, Narayana Jyoti, Ellestad Laura E, Prakobsaeng Nattiya, Porter Tom E
Abstract excerpt
Glucocorticoid (GC) treatment of rat or chicken embryonic pituitary (CEP) cells induces premature production of growth hormone (GH). GC induction of the GH gene requires ongoing protein synthesis, and the GH genes lack a canonical GC response element (GRE). To characterize cis-acting elements and identify trans-acting proteins involved in this process, we characterized the regulation of a luciferase reporter...
Topics
- Animals
- Cells, Cultured
- Chick Embryo
- Gene Deletion
- Gene Expression
- Glucocorticoids
- Growth Hormone
- Luciferases
- Models, Animal
- Mutation
- Pituitary Gland
- Promoter Regions, Genetic
- Proto-Oncogene Protein c-ets-1