Article
Site-specific conjugation of ScFvs antibodies to nanoparticles by bioorthogonal strain-promoted alkyne-nitrone cycloaddition.
Angewandte Chemie (International ed. in English) - 9 Jan 2012
Colombo Miriam, Sommaruga Silvia, Mazzucchelli Serena, Polito Laura, Verderio Paolo, Galeffi Patrizia, Corsi Fabio, Tortora Paolo, Prosperi Davide
Abstract excerpt
Particularly suitable: An N-terminal serine mutant of anti-HER2 scFv antibody was conjugated to polymer-coated magnetofluorescent nanoparticles by strain-promoted alkyne-nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2-positive breast cancer cells.
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