Article
Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthemi EC16.
Journal of bacteriology - 1 Sept 1990
He S Y, Collmer A
Abstract excerpt
The pehX gene encoding extracellular exo-poly-alpha-D-galacturonosidase (exoPG; EC 3.2.1.82) was isolated from a genomic library of the pectate lyase-deficient Erwinia chrysanthemi mutant UM1005 (a Nalr Kanr delta pelABCE derivative of EC16) by immunoscreening 2,800 Escherichia coli HB101 transfo...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- Erwinia
- Escherichia coli
- Genes, Bacterial
- Glycoside Hydrolases
- Immunoblotting
- Molecular Sequence Data
- Mutation
- Phenotype
- Restriction Mapping
- Virulence