Article
Differential effect of dsbA and dsbC mutations on extracellular enzyme secretion in Erwinia chrysanthemi.
Molecular microbiology - 1 May 1995
Shevchik V E, Bortoli-German I, Robert-Baudouy J, Robinet S, Barras F, Condemine G
Abstract excerpt
An Erwinia chrysanthemi gene able to complement an Escherichia coli dsbA mutation has been cloned and sequenced. This gene codes for a periplasmic protein with disulphide isomerase activity that has 69% identity and 94% similarity with the E. coli DsbA protein. An E. chrysanthemi dsbA-uidA fusion mutant has been constructed. dsbA expression seems to be constitutive. This mutant has multiple phenotypes resulting...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- Dickeya chrysanthemi
- Escherichia coli
- Extracellular Space
- Genes, Bacterial
- Genetic Complementation Test
- Isomerases
- Molecular Sequence Data
- Mutation