Article
Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes.
Journal of bacteriology - 1 Aug 1995
Alfano J R, Ham J H, Collmer A
Abstract excerpt
Erwinia chrysanthemi mutant CUCPB5047, delta(pelA pelE) delta(pelB pelC)::28bp delta(pelX) delta 4bp pehX::omega Cmr, was constructed, mutated with Tn5tac1, and screened for isopropyl-beta-D-thiogalactopyranoside-dependent pectate lyase (Pel) production. A Kmr SacI fragment from the hyperexpressi...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Dickeya chrysanthemi
- Gene Deletion
- Genes, Bacterial
- Isoenzymes
- Molecular Sequence Data
- Mutation
- Plasmids
- Polysaccharide-Lyases