Article
[Cloning, expression, isolation and properties of thymidine kinase herpes simplex virus, strain L2].
Bioorganicheskaia khimiia - 1 Jan 2000
Stepanenko V N, Esipov R S, Miroshnikov A I, Andronova V L, Galegov G A, Ias'ko M V, Gus'kova A V, Skoblov A Iu, Skoblov Iu S
Abstract excerpt
A thymidine kinase UL23 gene (EC 2.7.1.145) from an acyclovir-sensitive strain L2 of herpes simplex virus type 1 was cloned and expressed in E. coli. Enzyme was purified by chromatography to a homogeneous state controlled by PAG electrophoresis. The Michaelis constants for the reactions with thymidine and an acyclovir were determined. It was found that enzyme phosphorilate some modified nucleosides such as d2T,...
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