Efficient Conversion of Cane Molasses into Fructooligosaccharides by a Glucose Derepression Mutant of Aureobasidium melanogenum with High β-Fructofuranosidase Activity.

Abstract excerpt

Fructooligosaccharides (FOSs) are excellent food ingredients or feed additives by stimulating probiotics. In this paper, a CREA gene encoding a glucose repressor in the β-fructofuranosidase producer Aureobasidium melanogenum 33 with high-level FOS biosynthesis was disrupted, and glucose repression in disruptant D28 was relieved. The disruptant D28 produced up to 2100 U/mL of β-fructofuranosidase activity, whereas...

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