Article
Implementing multiplexed genotyping of non-small-cell lung cancers into routine clinical practice.
Annals of oncology : official journal of the European Society for Medical Oncology - 1 Dec 2011
Sequist L V, Heist R S, Shaw A T, Fidias P, Rosovsky R, Temel J S, Lennes I T, Digumarthy S, Waltman B A, Bast E, Tammireddy S, Morrissey L, Muzikansky A, Goldberg S B, Gainor J, Channick C L, Wain J C, Gaissert H, Donahue D M, Muniappan A, Wright C, Willers H, Mathisen D J, Choi N C, Baselga J, Lynch T J, Ellisen L W, Mino-Kenudson M, Lanuti M, Borger D R, Iafrate A J, Engelman J A, Dias-Santagata D
Abstract excerpt
BACKGROUND: Personalizing non-small-cell lung cancer (NSCLC) therapy toward oncogene addicted pathway inhibition is effective. Hence, the ability to determine a more comprehensive genotype for each case is becoming essential to optimal cancer care. METHODS: We developed a multiplexed PCR-based assay (SNaPshot) to simultaneously identify >50 mutations in several key NSCLC genes. SNaPshot and FISH for ALK...
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