Article
Using pyrosequencing to measure allele-specific mRNA abundance and infer the effects of cis- and trans-regulatory differences.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Wittkopp Patricia J
Abstract excerpt
Changes in gene expression are an important source of phenotypic differences within and between species. Differences in RNA abundance can be readily quantified between genotypes using a variety of tools, including microarrays, quantitative real-time PCR, cDNA sequencing, and in situ hybridization, but determining the genetic basis of heritable expression differences has historically been less straightforward....
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