Article
Structural analysis of proinsulin hexamer assembly by hydroxyl radical footprinting and computational modeling.
The Journal of biological chemistry - 23 Dec 2011
Kiselar Janna G, Datt Manish, Chance Mark R, Weiss Michael A
Abstract excerpt
Mutations in the insulin gene can impair proinsulin folding and cause diabetes mellitus. Although crystal structures of insulin dimers and hexamers are well established, proinsulin is refractory to crystallization. Although an NMR structure of an engineered proinsulin monomer has been reported, structures of the wild-type monomer and hexamer remain undetermined. We have utilized hydroxyl radical footprinting and...
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