Article
Structure of collagen receptor integrin α(1)I domain carrying the activating mutation E317A.
The Journal of biological chemistry - 16 Dec 2011
Lahti Matti, Bligt Eva, Niskanen Henri, Parkash Vimal, Brandt Anna-Maria, Jokinen Johanna, Patrikainen Pekka, Käpylä Jarmo, Heino Jyrki, Salminen Tiina A
Abstract excerpt
We have analyzed the structure and function of the integrin α(1)I domain harboring a gain-of-function mutation E317A. To promote protein crystallization, a double variant with an additional C139S mutation was used. In cell adhesion assays, the E317A mutation promoted binding to collagen. Similarly, the double mutation C139S/E317A increased adhesion compared with C139S alone. Furthermore, soluble α(1)I C139S/E317A...
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