Article
[Enrichment and screening of the microsatellite markers in Microtus fortis and its preliminary application in genetic diversity research].
Yi chuan = Hereditas - 1 Sept 2011
Ni Li-Ju, Tao Ling-Yun, Bai Xiong, Hu Jian-Hua, Gao Cheng, Xie Jian-Yun
Abstract excerpt
This study was to isolate microsatellite markers from Microtus fortis genome by magnetic beads enrichments. Through hybridization of biotin-labeled microsatellite oligonucleotide probes, which were captured by streptavidin-coated magnetic with the adaptor-ligated enzyme-digested genome fragments, single-stranded DNA fragments containing microsatellites were obtained. After PCR amplification, these fragments were...
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