Article
[Isolation of microsatellite markers from Paralichthys olivaceus and its application in genetic structure analysis].
Yi chuan = Hereditas - 1 Oct 2010
Wang Lei, Zhang Li-Dong, Wan Yu-Mei, Tan Zhao-Jun, Sun Xiao-Wen
Abstract excerpt
Magnetic bead enrich-method and plasmid detection-method were used to isolate microsatellite DNA from Paralichthys olivaceus genome. The microsatellite markers were applied to analyze natural population structure of four populations, which were collected from Dalian, Beidaihe, Dandong, and Qingdao. Two thousand eight hundred and five microsatellite sequence clones were isolated and 3120 microsatellite loci were...
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