Article
Portable microsatellite primers for Ficus (Moraceae).
American journal of botany - 1 May 2012
Garcia Marjorie, Bain Anthony, Tzeng Hsy-Yu, Peng Yan-Qiong, Chou Lien-Siang, Kjellberg Finn
Abstract excerpt
PREMISE OF THE STUDY: Highly portable microsatellite primers were developed for Ficus to facilitate investigation of genetic structure of complete regional floras using a single set of markers. METHODS AND RESULTS: Pyrosequencing of five species of Ficus produced a library of 5723 potential primers. Potential primers found in at least two species and presenting identical annealing temperatures were tested on a...
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