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Article

p-Thiophenylalanine-induced DNA cleavage and religation activity of a modified vaccinia topoisomerase IB.

Biochemistry - 1 Nov 2011

Chen Shengxi, Zhang Yi, Hecht Sidney M

Abstract excerpt

Vaccinia DNA topoisomerase IB is the smallest of the type IB topoisomerases. Because of its small size (314 amino acids) and target site specificity (5'(C/T)CCTTp(↓) sites), it constitutes an excellent model for studying the interaction of type IB enzymes with duplex DNA. In this study, p-thiophenylalanine was incorporated into the enzyme active site (position 274) by in vitro translation in the presence of a...

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Identifiers

PMID: 21942719
DOI: 10.1021/bi201291p

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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