Article
Mutation rate of bacteriophage ΦX174 modified through changes in GATC sequence context.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Oct 2011
Cuevas José M, Pereira-Gómez Marianoel, Sanjuán Rafael
Abstract excerpt
Bacteriophage ΦX174 has a relatively high mutation rate of 10⁻⁶ substitutions per nucleotide per strand copying. A thirty-fold reduction in the mutation rate was achieved by introducing seven GATC sequences in its genome. This motif allows for methyl-directed mismatch repair and is strongly avoided in nature by ΦX174 and other phages.
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