Article
Complementation of an Escherichia coli proC mutation by a gene cloned from Treponema pallidum.
Journal of bacteriology - 1 Jun 1990
Gherardini F C, Hobbs M M, Stamm L V, Bassford P J
Abstract excerpt
Little is known concerning the biosynthetic and metabolic capabilities of the syphilis agent, Treponema pallidum, because of the inability to cultivate continuously the organism in vitro. To circumvent the problem of cultivation, researchers have used recombinant DNA technology to express treponemal protein antigens in Escherichia coli. However, with a few notable exceptions, the specific cellular roles of these...
Topics
- Blotting, Southern
- Cloning, Molecular
- Escherichia coli
- Mutation
- Oxidoreductases Acting on CH-NH Group Donors
- Proline
- Pyrroline Carboxylate Reductases
- Recombinant Proteins
- Recombination, Genetic
- Treponema pallidum