Article
Identification and characterization of the Treponema pallidum tpn50 gene, an ompA homolog.
Infection and immunity - 1 Mar 1994
Hardham J M, Stamm L V
Abstract excerpt
Treponema pallidum is a pathogenic spirochete that has no known genetic exchange mechanisms. In order to identify treponemal genes encoding surface and secreted proteins, we carried out TnphoA mutagenesis of a T. pallidum genomic DNA library in Escherichia coli. Several of the resulting clones expressed enzymatically active T. pallidum-alkaline phosphatase fusion proteins. The DNA sequence of the 5' portion of a...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA
- Genes, Bacterial
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Recombinant Fusion Proteins