Article
Mutant alcohol dehydrogenase (ADH III) presequences that affect both in vitro mitochondrial import and in vitro processing by the matrix protease.
Molecular and cellular biology - 1 Jun 1990
Mooney D T, Pilgrim D B, Young E T
Abstract excerpt
Point mutations in the presequence of the mitochondrial alcohol dehydrogerase isoenzyme (ADH III) have been shown to affect either the import of the precursor protein into yeast mitochondria in vivo or its processing within the organelle. In the present work, the behavior of these mutants during in vitro import into isolated mitochondria was investigated. All point mutants tested were imported with a slower...
Topics
- Alcohol Dehydrogenase
- Amino Acid Sequence
- Base Sequence
- Enzyme Precursors
- Kinetics
- Mitochondria
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Peptide Hydrolases
- Protein Biosynthesis