Article
Cellular morphogenesis under stress is influenced by the sphingolipid pathway gene ISC1 and DNA integrity checkpoint genes in Saccharomyces cerevisiae.
Genetics - 1 Oct 2011
Tripathi Kaushlendra, Matmati Nabil, Zheng W Jim, Hannun Yusuf A, Mohanty Bidyut K
Abstract excerpt
In Saccharomyces cerevisiae, replication stress induced by hydroxyurea (HU) and methyl methanesulfonate (MMS) activates DNA integrity checkpoints; in checkpoint-defective yeast strains, HU treatment also induces morphological aberrations. We find that the sphingolipid pathway gene ISC1, the product of which catalyzes the generation of bioactive ceramides from complex sphingolipids, plays a novel role in...
Topics
- Actins
- Blotting, Western
- CDC28 Protein Kinase, S cerevisiae
- Cell Cycle Proteins
- Cell Wall
- Chitin
- DNA Damage
- DNA Repair
- DNA Replication
- DNA-Binding Proteins
- Hydroxyurea
- Methyl Methanesulfonate
- Microscopy, Fluorescence