Article
Identification of committed mononuclear precursors for osteoclast-like cells formed in long term human marrow cultures.
Endocrinology - 1 May 1990
Kurihara N, Chenu C, Miller M, Civin C, Roodman G D
Abstract excerpt
Nonadherent marrow mononuclear cells enriched for hematopoietic progenitor cells were cultured in semisolid medium with recombinant human granulocyte-macrophage colony-stimulating factor for 9 days to form colony forming unit-granulocyte macrophage (CFU-GM) colonies. 1,25-Dihydroxyvitamin D was then gently layered over the cultures. After 2 weeks, approximately 30% of the colonies that formed were composed of...
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