Article
De novo assembly and characterization of the carrot transcriptome reveals novel genes, new markers, and genetic diversity.
BMC genomics - 2 Aug 2011
Iorizzo Massimo, Senalik Douglas A, Grzebelus Dariusz, Bowman Megan, Cavagnaro Pablo F, Matvienko Marta, Ashrafi Hamid, Van Deynze Allen, Simon Philipp W
Abstract excerpt
BACKGROUND: Among next generation sequence technologies, platforms such as Illumina and SOLiD produce short reads but with higher coverage and lower cost per sequenced nucleotide than 454 or Sanger. A challenge now is to develop efficient strategies to use short-read length platforms for de novo assembly and marker development. The scope of this study was to develop a de novo assembly of carrot ESTs from multiple...
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