Article
Supercondensed structure of plasmid pBR322 DNA in an Escherichia coli DNA topoisomerase II mutant.
Journal of molecular biology - 20 Nov 1990
Huang X T, Chen X
Abstract excerpt
An unusual structural component, supercondensed pBR322 DNA, has been found in plasmid pBR322 DNA samples isolated from a DNA topoisomerase II mutant of Escherichia coli, SD108 (topA+, gyrB225). The supercondensed pBR322 DNA moved faster than supercoiled pBR322 DNA as a homogeneous band in agrose gels when the DNA samples were analysed by electrophoresis. The mobility of the supercondensed DNA was not...
Topics
- DNA Topoisomerases, Type II
- DNA, Bacterial
- Electrophoresis, Agar Gel
- Escherichia coli
- Microscopy, Electron
- Mutation
- Plasmids