Article
Infection of Epstein-Barr virus-transformed lymphoblastoid B cells by the human immunodeficiency virus: evidence for a persistent and productive infection leading to B cell phenotypic changes.
European journal of immunology - 1 Sept 1990
De Rossi A, Roncella S, Calabro M L, D'Andrea E, Pasti M, Panozzo M, Mammano F, Ferrarini M, Chieco-Bianchi L
Abstract excerpt
Four Epstein-Barr virus-positive lymphoblastoid cell lines (LCL) were successfully infected in vitro with immunodeficiency virus type 1 (HIV-1) as demonstrated by reverse transcriptase activity and p24 HIV antigen in culture supernatants, positive cell staining for gag-encoded HIV proteins, presence of viral HIV genome by Southern blot analysis and ulstrastructural observations. In addition, both HIV-1-infected B...
Topics
- Antigens, Differentiation, B-Lymphocyte
- B-Lymphocytes
- CD4 Antigens
- Cell Line
- Cell Transformation, Viral
- Genotype
- HIV Infections
- HIV-1
- Herpesvirus 4, Human
- Humans