Buffer enhancement of proton transfer in catalysis by human carbonic anhydrase III.

doi:10.1021/bi00479a009

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Article

Buffer enhancement of proton transfer in catalysis by human carbonic anhydrase III.

Biochemistry - 10 Jul 1990

Tu C K, Paranawithana S R, Jewell D A, Tanhauser S M, LoGrasso P V, Wynns G C, Laipis P J, Silverman D N

Abstract excerpt

Among the isozymes of carbonic anhydrase, isozyme III is the least efficient in the catalysis of the hydration of CO2 and was previously thought to be unaffected by proton transfer from buffers to the active site. We report that buffers of small size, especially imidazole, increase the rate of ca...

Topics

Binding Sites
Buffers
Carbon Dioxide
Carbonic Anhydrases
Catalysis
Humans
Imidazoles
Kinetics
Molecular Weight
Mutation
Protons
Recombinant Proteins

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