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Article

Activation of carbonic anhydrase II by active-site incorporation of histidine analogs.

Archives of biochemistry and biophysics - 15 Jan 2004

Elder Ileana, Han Shoufa, Tu Chingkuang, Steele Heather, Laipis Philip J, Viola Ronald E, Silverman David N

Abstract excerpt

The hydration of CO2 catalyzed by human carbonic anhydrase II (HCA II) is accompanied by proton transfer from the zinc-bound water of the enzyme to solution. We have replaced the proton shuttling residue His 64 with Ala and placed cysteine residues within the active-site cavity by mutating sites Trp 5, Asn 62, Ile 91, and Phe 131. These mutants were modified at the single inserted cysteine with imidazole analogs...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 14984209
DOI: 10.1016/j.abb.2003.11.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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