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Article

Proton transfer in a Thr200His mutant of human carbonic anhydrase II.

Proteins - 1 Nov 2005

Bhatt Deepa, Tu Chingkuang, Fisher S Zoë, Hernandez Prada Jose A, McKenna Robert, Silverman David N

Abstract excerpt

Human carbonic anhydrase II (HCA II) has a histidine at position 64 (His64) that donates a proton to the zinc-bound hydroxide in catalysis of the dehydration of bicarbonate. To examine the effect of the histidine location on proton shuttling, His64 was replaced with Ala and Thr200 replaced with histidine (H64A-T200H HCAII), effectively relocating the proton shuttle residue 2 A closer to the zinc-bound hydroxide...

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Identifiers

PMID: 16106378
DOI: 10.1002/prot.20615

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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