Article
Molecular insights into miRNA processing by Arabidopsis thaliana SERRATE.
Nucleic acids research - 1 Sept 2011
Machida Satoru, Chen Hong-Ying, Adam Yuan Y
Abstract excerpt
In plant, primary transcripts (pri-miRNAs) transcribed from miRNA genes by RNA polymerase II are first processed into stem-loop pre-miRNAs and further chopped into ∼21 nt long miRNAs by RNase III-like enzyme DCL1. SERRATE (SE) protein is an essential component for miRNA processing by assisting DCL1 for accurate cleavage. Here we report the crystal structure of Arabidopsis SE core (residues 194-543) at 2.7 Å. SE...
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