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Article

Cloning, characterization, and complementation of lesions causing acid sensitivity in Tn5-induced mutants of Rhizobium meliloti WSM419.

Journal of bacteriology - 1 Sept 1990

Goss T J, O'Hara G W, Dilworth M J, Glenn A R

Abstract excerpt

Four Tn5-induced mutants of Rhizobium meliloti WSM419 were unable to grow or maintain intracellular pH at an external pH of 5.6. Restriction analysis of DNA fragments carrying Tn5 and flanking sequences cloned from these mutants indicated that all four cloned mutations are unique and that the two strains (TG1-6 and TG1-11) carry Tn5 insertions which are within 4.4 kilobases of each other on a single EcoRI...

Topics

Cloning, Molecular
Cosmids
DNA Probes
DNA Transposable Elements
DNA, Bacterial
Escherichia coli
Genetic Complementation Test
Genotype
Hydrogen-Ion Concentration
Mutation
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Restriction Mapping

Rhizobium

Transduction, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 2168374
DOI: 10.1128/jb.172.9.5173-5179.1990
PMCID: PMC213178

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2168374-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cloning, characterization, and complementation of lesions causing acid sensitivity in Tn5-induced mutants of Rhizobium meliloti WSM419.PMID 2168374Read ↗

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