Article
The pha gene cluster of Rhizobium meliloti involved in pH adaptation and symbiosis encodes a novel type of K+ efflux system.
Molecular microbiology - 1 Jun 1998
Putnoky P, Kereszt A, Nakamura T, Endre G, Grosskopf E, Kiss P, Kondorosi A
Abstract excerpt
The fix-2 mutant of Rhizobium meliloti affected in the invasion of alfalfa root nodules (Inf-/Fix-) is K+ sensitive and unable to adapt to alkaline pH in the presence of K+. Using directed Tn5 mutagenesis, we delimited a 6kb genomic region in which mutations resulted in both Inf-/Fix- and K+-sensitive phenotypes. In this DNA region, seven open reading frames (ORFs) were identified and the corresponding genes were...
Topics
- Adaptation, Physiological
- Alkaline Phosphatase
- Bacterial Proteins
- Codon, Initiator
- DNA Transposable Elements
- Genes, Bacterial
- Hydrogen-Ion Concentration
- Ion Transport
- Membrane Proteins
- Molecular Sequence Data
- Multigene Family
- Mutagenesis
- Mutation