Isolation of salt-sensitive mutants from Sinorhizobium meliloti and characterization of genes involved in salt tolerance.

doi:10.1111/j.1472-765X.2004.01577.x

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Article

Isolation of salt-sensitive mutants from Sinorhizobium meliloti and characterization of genes involved in salt tolerance.

Letters in applied microbiology - 1 Jan 2004

Wei W, Jiang J, Li X, Wang L, Yang S S

Abstract excerpt

AIMS: The purpose of our research is to isolate salt-sensitive mutants and to study the genes involved in salt tolerance of the salt-tolerant bacterium Sinorhizobium meliloti 042BM. METHODS: Wild type S. meliloti 042BM bacteria are able to grow at a NaCl concentration of 0.6 mol l(-1). A transposon Tn5-1063a mutagenesis library of S. meliloti 042BM was constructed and eight salt-sensitive mutants were isolated,...

Topics

Bacterial Proteins
Culture Media
DNA Transposable Elements
Gene Expression Regulation, Bacterial
Medicago sativa
Molecular Sequence Data
Mutagenesis, Insertional
Mutation
Osmotic Pressure
Sequence Analysis, DNA
Sinorhizobium meliloti
Sodium Chloride

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Open in PubMed

Identifiers

PMID: 15287875
DOI: 10.1111/j.1472-765X.2004.01577.x

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