Article
Analysis of a 1600-kilobase Rhizobium meliloti megaplasmid using defined deletions generated in vivo.
Genetics - 1 Jan 1991
Charles T C, Finan T M
Abstract excerpt
A series of 120-600 kilobase deletions with defined endpoints were made in the 1600-kilobase Rhizobium meliloti megaplasmid pRmeSU47b, by homologous recombination between the IS50 elements of transposon insertions. Utilizing IS 50-mediated homologous recombination we also made defined reductions in deletion size and combined adjacent deletions. Deletion structure was confirmed by phage transduction and Southern...
Topics
- Blotting, Southern
- Chromosome Deletion
- Chromosome Mapping
- Chromosomes, Bacterial
- DNA Transposable Elements
- DNA, Bacterial
- DNA, Recombinant
- Electrophoresis, Agar Gel
- Mutagenesis
- Phenotype
- Plants