Article
A Single phenylalanine residue in the main intracellular loop of α1 γ-aminobutyric acid type A and glycine receptors influences their sensitivity to propofol.
Anesthesiology - 1 Sept 2011
Moraga-Cid Gustavo, Yevenes Gonzalo E, Schmalzing Günther, Peoples Robert W, Aguayo Luis G
Abstract excerpt
BACKGROUND: The intravenous anesthetic propofol acts as a positive allosteric modulator of glycine (GlyRs) and γ-aminobutyric acid type A (GABAARs) receptors. Although the role of transmembrane residues is recognized, little is known about the involvement of other regions in the modulatory effects of propofol. Therefore, the influence of the large intracellular loop in propofol sensitivity of both receptors was...
Topics
- Alanine
- Amino Acid Sequence
- Amino Acid Substitution
- Anesthetics, Intravenous
- DNA
- Electrophysiological Phenomena
- Extracellular Space
- HEK293 Cells
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Patch-Clamp Techniques