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Article

Quantitative polymerase chain reaction analysis of herpes simplex virus DNA in ganglia of mice infected with replication-incompetent mutants.

Journal of virology - 1 Sept 1990

Katz J P, Bodin E T, Coen D M

Abstract excerpt

To study the roles of viral genes in the establishment and maintenance of herpes simplex virus (HSV) latency, we have developed a polymerase chain reaction assay that is both quantitative and sensitive. Using this assay, we analyzed the levels of viral DNA in trigeminal ganglia of mice inoculated corneally with HSV mutants that are defective for virus replication at one or more sites in mice and for reactivation...

Topics

Animals
Base Sequence
DNA Replication
DNA, Viral
Genes, Viral
Herpes Simplex
Mice
Mice, Inbred Strains
Molecular Sequence Data
Mutation
Nucleic Acid Amplification Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oligonucleotide Probes

Polymerase Chain Reaction

Simplexvirus

Trigeminal Ganglion

Source

Open in PubMed Open full text

Identifiers

PMID: 2166818
DOI: 10.1128/JVI.64.9.4288-4295.1990
PMCID: PMC247895

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2166818-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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