Article
Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants.
Science (New York, N.Y.) - 1 Jun 1990
Altenbach C, Marti T, Khorana H G, Hubbell W L
Abstract excerpt
Transmembrane proteins serve important biological functions, yet precise information on their secondary and tertiary structure is very limited. The boundaries and structures of membrane-embedded domains in integral membrane proteins can be determined by a method based on a combination of site-specific mutagenesis and nitroxide spin labeling. The application to one polypeptide segment in bacteriorhodopsin, a...
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