Article
Construction and analysis of TnphoA mutants of enteropathogenic Escherichia coli unable to invade HEp-2 cells.
Infection and immunity - 1 Jun 1990
Donnenberg M S, Calderwood S B, Donohue-Rolfe A, Keusch G T, Kaper J B
Abstract excerpt
Enteropathogenic Escherichia coli (EPEC) strains have recently been shown to invade tissue culture cells. We describe a set of 22 Tn5 IS50L::phoA (TnphoA) insertion mutants of EPEC strain E2348-69 that are unable to invade HEp-2 cells. Each mutant was tested for the ability to adhere to and to in...
Topics
- Actin Cytoskeleton
- Actins
- Bacterial Adhesion
- Blotting, Southern
- Cells, Cultured
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Humans
- In Vitro Techniques
- Microscopy, Fluorescence
- Mutation
- Restriction Mapping