RecA-ssDNA interaction: induced strand cleavage by hydroxyl radical at a defined distance from the 5' end.

doi:10.1007/BF00391753

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Article

RecA-ssDNA interaction: induced strand cleavage by hydroxyl radical at a defined distance from the 5' end.

Molecular & general genetics : MGG - 1 Feb 1990

Akaboshi E, Howard-Flanders P

Abstract excerpt

Interaction of the RecA protein with single-stranded DNA (ssDNA) was analyzed by challenge with the hydroxyl radical, which can cleave the DNA backbone. We found that RecA protein induces cleavage by the radical at a defined distance from the 5' end. The cleavage was at the 11th nucleotide in man...

Topics

Adenosine Triphosphate
Base Sequence
DNA, Single-Stranded
Free Radicals
Hydroxides
Hydroxyl Radical
Molecular Sequence Data
Mutation
Oligodeoxyribonucleotides
Rec A Recombinases

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