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Isolation of a mutant auxotrophic for L-alanine and identification of three major aminotransferases that synthesize L-alanine in Escherichia coli.

Bioscience, biotechnology, and biochemistry - 1 Jan 2011

Yoneyama Hiroshi, Hori Hatsuhiro, Lim Sang-Jo, Murata Tatsuya, Ando Tasuke, Isogai Emiko, Katsumata Ryoichi

Abstract excerpt

For Escherichia coli, it has been assumed that L-alanine is synthesized by alanine-valine transaminase (AvtA) in conjunction with an unknown alanine aminotransferase(s). We isolated alanine auxotrophs from a prototrophic double mutant deficient in AvtA and YfbQ, a novel alanine aminotransferase, by chemical mutagenesis. A shotgun cloning experiment identified two genes, uncharacterized yfdZ and serC, that...

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PMID: 21597182
DOI: 10.1271/bbb.100905

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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