Large-scale detection of rare variants via pooled multiplexed next-generation sequencing: towards next-generation Ecotilling.

Abstract excerpt

Common variants, such as those identified by genome-wide association scans, explain only a small proportion of trait variation. Growing evidence suggests that rare functional variants, which are usually missed by genome-wide association scans, play an important role in determining the phenotype. We used pooled multiplexed next-generation sequencing and a customized analysis workflow to detect mutations in five...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Chromosome Mapping
• Share to Chromosomes, Plant