Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Lentivirus Production Is Influenced by SV40 Large T-Antigen and Chromosomal Integration of the Vector in HEK293 Cells

9 May 2011

Abstract excerpt

Currently, lentiviral vectors for research and gene therapy are produced from 293-T cells that are transiently transfected with plasmids encoding the vector and helper functions. However, transiently transfected vectors as well as the presence of SV40 virus large T-antigen (T-Ag) cause serious technical and safety considerations. We aimed to exploit single copy integration sites in the HEK293 genome supporting...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR and Genetic Engineering
Share to Viral Infections and Immunology Research
Share to Virus-based gene therapy research

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 21554103
DOI: 10.1089/hum.2010.143

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Lentivirus Production Is Influenced by SV40 Large T-Antigen and Chromosomal Integration of the Vector in HEK293 CellsPMID 21554103Read ↗

Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding RegionSemantic proximity Read ↗

Production of Lentiviral Vectors Using Suspension Cells Grown in Serum-free MediaSemantic proximity Read ↗

Construction of stable packaging cell lines for clinical lentiviral vector productionSemantic proximity Read ↗

Development of Lentiviral Packaging Cells and Scale Up of Production to Meet the Growing Demand in Cell and Gene TherapySemantic proximity Read ↗

Production of lentiviral vectorsSemantic proximity Read ↗

A new T-antigen negative HEK293 cell line with improved AAV productivitySemantic proximity Read ↗

Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate ImmunitySemantic proximity Read ↗

A New-Generation Stable Inducible Packaging Cell Line for Lentiviral VectorsSemantic proximity Read ↗

Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA ConstructSemantic proximity Read ↗

Impact of the size of CRISPR-Cas gene cassettes on the packaging efficiency and transduction titer of lentiviral vectorsSemantic proximity Read ↗

Generation of stable suspension producer cell lines for serum‐free lentivirus productionSemantic proximity Read ↗

Development of Stable Packaging and Producer Cell Lines for the Production of AAV VectorsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.