The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes.

doi:10.1099/0022-1317-71-1-173

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes.

The Journal of general virology - 1 Jan 1990

Snijders P J, van den Brule A J, Schrijnemakers H F, Snow G, Meijer C J, Walboomers J M

Abstract excerpt

A novel polymerase chain reaction (PCR) method was developed that permits the detection of 11 different human papillomavirus (HPV) genotypes using two general primer sets. By computer-assisted sequence analysis, two pairs of general primers were selected from the conserved L1 open reading frame and tested in the PCR on a set of cloned HPV genotypes. Experimental analysis showed that up to three mismatches between...

Topics

Base Sequence
Blotting, Southern
Carcinoma, Squamous Cell
DNA, Viral
Female
Gene Amplification
Genotype
HeLa Cells
Humans
Larynx
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleic Acid Hybridization

Papillomaviridae

Polymerase Chain Reaction

Restriction Mapping

Tumor Cells, Cultured

Uterine Cervical Neoplasms

Source

Open in PubMed

Identifiers

PMID: 2154534
DOI: 10.1099/0022-1317-71-1-173

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2154534-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes.PMID 2154534Read ↗

Modified General Primer PCR System for Sensitive Detection of Multiple Types of Oncogenic Human PapillomavirusSemantic proximity Read ↗

A novel human papillomavirus sequence based on L1 general primers.Semantic proximity Read ↗

Group-specific differentiation between high- and low-risk human papillomavirus genotypes by general primer-mediated PCR and two cocktails of oligonucleotide probes.Semantic proximity Read ↗

General primer‐mediated polymerase chain reaction permits the detection of sequenced and still unsequenced human papillomavirus genotypes in cervical scrapes and carcinomasSemantic proximity Read ↗

Identification and Assessment Of Known And Novel Human Papillomaviruses by Polymerase Chain Reaction Amplification, Restriction Fragment Length Polymorphisms, Nucleotide Sequence, and Phylogenetic AlgorithmsSemantic proximity Read ↗

A novel strategy for human papillomavirus detection and genotyping with SybrGreen and molecular beacon polymerase chain reaction.Semantic proximity Read ↗

Multiplex PCR assay for the rapid identification of human papillomavirus genotypes 16, 18, 45, 35, 66, 33, 51, 58, and 31 in clinical samples.Semantic proximity Read ↗

Evaluation of combined general primer-mediated PCR sequencing and type-specific PCR strategies for determination of human papillomavirus genotypes in cervical cell specimens.Semantic proximity Read ↗

Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66.Semantic proximity Read ↗

A novel and rapid PCR-based method for genotyping human papillomaviruses in clinical samples.Semantic proximity Read ↗

A broad range of human papillomavirus types detected with a general PCR method suitable for analysis of cutaneous tumours and normal skinSemantic proximity Read ↗

Comparison of human papillomavirus detection and genotyping with four different prime sets by PCR-sequencing.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.