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Article

The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes.

The Journal of general virology - 1 Jan 1990

Snijders P J, van den Brule A J, Schrijnemakers H F, Snow G, Meijer C J, Walboomers J M

Abstract excerpt

A novel polymerase chain reaction (PCR) method was developed that permits the detection of 11 different human papillomavirus (HPV) genotypes using two general primer sets. By computer-assisted sequence analysis, two pairs of general primers were selected from the conserved L1 open reading frame and tested in the PCR on a set of cloned HPV genotypes. Experimental analysis showed that up to three mismatches between...

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PMID: 2154534
DOI: 10.1099/0022-1317-71-1-173

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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