A broad range of human papillomavirus types detected with a general PCR method suitable for analysis of cutaneous tumours and normal skin

doi:10.1099/0022-1317-80-9-2437

Article

A broad range of human papillomavirus types detected with a general PCR method suitable for analysis of cutaneous tumours and normal skin

1 Sept 1999

Abstract excerpt

A pair of degenerate PCR primers (FAP59/64) was designed from two relatively conserved regions of the L1 open reading frame of most human papillomaviruses (HPV). The size of the generated amplicon was about 480 bp. PCR using these primers was found capable of amplifying DNA from 87% (65/75) of the HPV types tested, its sensitivity being 1-10 copies for HPV-5, -20 and -30 clones. HPV was found in 63% (5/8) of...

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