Article
The N-terminal domain of the insertion sequence 30 transposase interacts specifically with the terminal inverted repeats of the element.
The Journal of biological chemistry - 5 Mar 1990
Stalder R, Caspers P, Olasz F, Arber W
Abstract excerpt
The gene for the insertion sequence (IS) 30 transposase is placed under the control of the tac promoter, and large quantities of transposase are expressed upon induction. The resulting protein precipitates inside the Escherichia coli cells in the form of inclusion bodies which, upon cell lysis, cannot be dissolved under nondenaturing conditions. In contrast, the N-terminal third of the transposase, a 17-kDa...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli
- Gene Expression
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Oligonucleotide Probes