Article
Random mutants of a Pleurotus ostreatus laccase as new biocatalysts for industrial effluents bioremediation.
Journal of applied microbiology - 1 Mar 2010
Miele A, Giardina P, Sannia G, Faraco V
Abstract excerpt
AIMS: To select better performing laccase variants among the 2300 randomly mutated variants of Pleurotus ostreatus POXA1b laccase to develop improved laccase-based biocatalysts. METHODS AND RESULTS: Screening of collections of 2300 randomly mutated variants of POXA1b was performed by assaying activity towards the phenolic substrate 2,6-dimethoxyphenol. Two new variants endowed with higher enzyme activity than the...
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