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Article

[Enzymatic activities of recombinant human NAMPT and NAMPT (H247A) proteins].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences - 1 Mar 2011

Wang Feng, Huang Peng, Liu Zhu, Lu Yun-Bi, Wei Er-Qing, Zhang Wei-Ping, Tang Chun

Abstract excerpt

OBJECTIVE: To prepare and purify recombinant human NAMPT and NAMPT (H247A) proteins and to detect their enzymatic activity. METHODS: Using pcDNA3.1-hnampt as template, full-length hnampt was sub-cloned into pET-11a(+) plasmid. The hnampt (H247A) mutant was obtained by site-directed mutagenesis. The plasmids were introduced in Escherichia coli BL21star for protein expression. The recombined NAMPT and NAMPT (H247A)...

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Identifiers

PMID: 21488211
DOI: 10.3785/j.issn.1008-9292.2011.02.007

Genes

NAMPT
SDS

Variants

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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