Use of different molecular typing methods for the study of heterogeneity within Clostridium difficile toxinotypes V and III.

Abstract excerpt

Clostridium difficile strains of toxinotypes III (n = 13) and V (n = 45) were typed by agarose gel-based PCR ribotyping, capillary gel electrophoresis-based PCR ribotyping and PFGE using two different restriction enzymes, SmaI and SacII. With conventional agarose gel-based PCR ribotyping, toxinotype III strains were distributed among six different PCR ribotypes and toxinotype V strains into three different PCR...

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